Gel Electrophoresis is a method to seperate DNAs or proteins by an electrical charge through a gel, thus the name.
DNA is a negatively charged molecule, thus it is moved by electric current from the negative end to the positive end. DNA moves in agarose that works like a sieve. The DNA is then seperated according to their size, the smaller or shorter fragments will move faster towards the postive charged end.
A video on how agarose gel electrophoresis is performed:
Proteins are commonly seperated by using polyacrylamide gel electrophoresis (PAGE) to characterize individual proteins in a complex sample or to examine multiple proteins within a single sample.
For more info on Polyacrylamide Gel Electrophoresis
The electrophoresis procedure can be a starting point for future additional identification and isolation of DNA fragments.
An example of 1D gel electrophoresis:
PS: Click the image if it's not moving.
An example of 2D gel electrophoresis:
Current projects:
From the University of Bristol, Proteomics Facility.
One example is Protein Expression Analysis.
Objective: To be able to elucidate proteins which may be downstream of galanin and therefore play important trophic roles following nerve injury.
More!
2D Gel Matching
Department of Computer Science, Free University of Berlin
Basically, this project is to identify protein information on a gel image and compare it with another gel image which already has the information on the identity of the proteins is performed.
Visit here for more information.
1 comment:
how about gel electrophoresis on protein? It is an important technique in proteomics.
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