ICAT reagents were developed by Professor Aebersold at the University of Washington. Researchers can use ICAT to compare relative protein abundance between two samples, often this may be between healthy and diseased tissue, but may be one of any number of comparisons. The advantage of this technology over gel electrophoresis is its speed and its ease of automation.
Recent Project using ICAT
Here some some project that uses ICAT:
- Complementary analysis of the Mycobacterium tuberculosis proteome by two dimensional electrophoresis and isotope coded affinity tag technology
This project was to show that using different type of technologies to the same sample would provide different type of result. And to help allivate the limitation of two-Dimensional electrophoresis and mass spectrometric identifcation method, ICAT was used.
source: http://www.proteomecenter.org/PDFs/Schmidt.Complementary_analysis.MCP.o3.pdf - Isotope coded affinity tag (ICAT)-based protein profiling
The ICAT reagent was designed to affinity isolate and quantify via the use of a stable isotope the relative concentrations of cysteine-containing tryptic peptides obtained from digests of control versus experimental samples.
Here are some project that using the ICAT based protein profiling:
http://keck.med.yale.edu/prochem/icat/references.htm
source:
http://keck.med.yale.edu/prochem/icat/
My thoughts:
ICAT is often used for protein analysis as it can be used to Quantitative profiling which can help to prostate cancer cells which in the future, may help to discover a definate cure for cancer which would be a great breakthrough in the medical realm.
other reference sources:
http://www.chemsoc.org/ExemplarChem/entries/2002/proteomics/icat.htm
http://www3.interscience.wiley.com/cgi-bin/abstract/107614589/ABSTRACT
No comments:
Post a Comment